Dufferin OPP are investigating a single-vehicle crash north of Shelburne.
The crash occurred on Highway 10 in Melancthon Township Monday afternoon.
Two people inside the vehicle were taken to a Toronto hospital with serious injuries.
Highway 10 between 240 Sideroad and 250 Sideroad was closed for several hours as the Technical Traffic Collision Investigation (TTCI) unit along with the OPP Identification Unit, and the Nottawasaga Crime Unit were determining the cause of the crash.
Investigators are asking for anyone who may have witnessed this crash to contact OPP or Crime Stoppers.